DNA Sequencing Request Form

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S.#	Code Name for Template	Temp. Type	Temp. Conc.	Fragment Size	Primer Name & Tm	Primer Conc.	Status
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Instructions

Template Concentration Table

Enter only one template/primer per row
Templates should be provided at the concentrations as given in the table below. At least 5.0 ul will be required per sequencing reaction.
Primers should be provided at a concentration of 3.2 pico moles/ul. 5.0 ul will be required per sequencing reaction.
Provide highly purified DNA template. Attach gel photograph with this form.
It is certified that there is no biological hazardous material in the samples.

Plasmid	300 ng/ul	PCR Fragment 100-200 bp	3 ng/ul
Cosmid	1 ug/ul	PCR Fragment 200-500 bp	10 ng/ul
BAC	1 ug/ul	PCR Fragment 500-1000 bp	20 ng/ul
Bacterial Genomic	3 ug/ul	PCR Fragment 1000-2000 bp	40 ng/ul
Phage	1 ug/ul	PCR Fragment ≥2000 bp	100 ng/ul

Need Help with Primer Picking?

If you need assistance in selecting primers for your research, you can use the Primer3Plus tool. This tool allows you to pick primers from a DNA sequence with various customizable settings. Whether you’re working on detection, PCR, or other applications, Primer3Plus can help you find the right primers for your project.

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